Centre de Référence Maladies Rares


Biotherapy of genetic diseases, inflammatory disorders and cancers (BMGIC)

FR: Biologie Fondamentale Appliquée à la Médecine

U1035Display navigation New Technics FR / EN

New technics

Cell culture platform

  • Culture cells from skin biopsies: fibroblasts, keratinocytes, melanocytes, cell …
  • epidermis 3D reconstruction, melanised or not

Platform histology and immunohistochemistry

  • Fixation, paraffin embedding, cutting and assembly of blades
  • Standard stains (hematoxylin-eosin) and specific (Fontana-Masson trichrome Masson …)
  • Immunohistochemical staining (Dako Autostainer and PT_Link controllers)
  • Optical and epifluorescent microscope, standard and reversed, with acquisition of digital images

Platform transcriptomic

  • QPCR and RTQPCR analysis (Stratagene Mx3000P)

Metabolism study

  • Biochemical analyzer for measurement of metabolism (glucose, lactate, glutamine …)
  • Oxygraph for measuring the oxygen consumption
  • Thermo Scientific Multiskan EX spectrophotometer

Study of cellular response to irradiation

  • Cell Irradiation UVA-UVB-UVC
  • small animals Irradiation UVA-UVB

Western blot

  • Electrophoresis and transfer onto PVDF / nitrocellulose by semi-dry or liquid
  • Picture acquisition by scanning (Fuji Player LAS3000)

Flow cytometer (BD FACS Canto II)

INSERM U1035 - University of Bordeaux
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