Centre de Référence Maladies Rares

U1035 INSERM

Biotherapy of genetic diseases, inflammatory disorders and cancers (BMGIC)

FR: Biologie Fondamentale Appliquée à la Médecine

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Xenopus core facility
Project X1

Xenopus Core Facility

Head:

Pierre THIÉBAUD
Research Scientist, Head of xenopus platform

The platform is:

keywords: xenopus, oocyte, embryo, microinjection, pluripotent cells, transgenesis, disease modeling

Description:

Our platform is based on the Xenopus facility recently established at the University of Bordeaux and integrated in the “Centre Broca Nouvelle-Aquitaine”. Animals are provided by the “Centre de Ressources Biologiques” of the University of Rennes (http://xenopus.univ-rennes1.fr/).
The platform is located in the INSERM unit 1035 and provides space and equipment for experimentation on oocyte and embryo. We have 3 injection devices with one equipped for images capture. A fluorescent stereomicrosope allows the observation of transgenic animals. We can perform on demand experiments as collaborations or pay for service.

  • Our platform is intended to provide research teams with the Xenopus embryo model. This model constitutes a powerful vertebrate in vivo model to explore the basic mechanisms of cellular functions. It allows the study not only of the function and regulation of genes but also of the fundamental cellular processes such as proliferation, differentiation, migration and apoptosis. The approaches used rely on gain and loss of function that allow human diseases modeling.
  • Pluripotent cells (animal cap cells) can be derived from the embryo and can be induced to differentiate into a variety of tissues belonging to the three germ layers.
  • The different techniques we use are mRNA, DNA or antisense morpholinos oligonucelotides injection in embryo. Gene expression analysis are based on in situ hybridization and immunohistochemistry.
  • CRISPR-CAS9 strategy can be implemented if necessary.

Leaders contacts

  • scientific : pierre.thiebaud@u-bordeaux.fr / 05 57 57 92 65
  • technical : sandrine.fedou@u-bordeaux.fr / 05 57 57 92 65

Related publications:

  • Vestigial-like 3 is a novel Ets1 interacting partner and regulates trigeminal nerve formation and cranial neural crest migration. Simon E, Thézé N, Fédou S, Thiébaud P and Faucheux C Biology Open (2017-10) 6:1528-1540
  • From vestigial to vestigial-like: the Drosophila gene that has taken wing Simon E, Faucheux C, Zider A, Thézé N and Thiébaud P. Dev Genes & Evol (2016-07) 226:297-315
  • Overexpression of Leap2 impairs Xenopus embryonic development and modulates FGF and activin signals. Thiébaud P, Garbay B, Auguste P, Sénéchal CL, Maciejewska Z, Fédou S., Gauthereau X, Costaglioli P and Thézé N. Peptides (2016-06) 83:21-8
  • MRAS GTPase is a novel stemness marker that impacts mouse embryonic stem cell plasticity and Xenopus embryonic cell fate Mathieu ME, Faucheux C, Saucourt C, Soulet F, Gauthereau X, Fédou S, Trouillas M, Thézé N, Thiébaud P* and Boeuf H*. * co-corresponding authors. Development (2013-08) 140, 3311-3322
  • Comparative functional analysis of ZFP36 genes during Xenopus development. Tréguer K, Faucheux C, Veschambre P, Fédou S, Thézé N and Thiébaud P. PloS One (2013-01) 8, e54550
  • WD repeat-containing protein 5, a ubiquitously expressed histone methyltransferase adaptor protein, regulates smooth muscle cell-selective gene activation through interaction with pituitary homeobox 2. Gan Q, Thiébaud P, Thézé N, Jin L, Xu G, Grant P and Owens GK. J Biol Chem (2011-06) 286, 21853-21864
  • Vestigial like gene family expression in Xenopus: common and divergent features with other vertebrates. Faucheux C, Naye F, Tréguer K, Fédou S, Thiébaud P and Thézé, N. Int J Dev Biol (2010) 54, 1375-1382.

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INSERM U1035! - University of Bordeaux
Work
146 rue Léo Saignat - PE building south zone, 4th floor
33000 Bordeaux
Aquitaine
France
Work +33 557 57 13 73/74
sarah.lesjean@inserm.fr